Senior WHO official Bruce Aylward told the BBC that risk assessment plans were in place, and reiterated that there was no need to delay the Games.
The mayor of Rio said disease-carrying mosquitoes were being eradicated.
The officials were responding to an open letter by scientists saying it was "unethical" for the Games to go ahead.
The letter also said the global health body should revisit its Zika guidance.
The Zika virus is linked to severe birth defects.
Between February and April, Brazil registered more than 90,000 likely cases of Zika. The number of babies born with Zika-linked defects stood at 4,908 in April.
Dr Aylward, who heads the WHO's emergency programme, told the BBC that it was already carrying out a risk-assessment programme "about this disease and the risks it poses both to individuals who get and those who might be subsequently exposed".
In addition, he said, independent experts had reported to the WHO on the implications of the outbreak for travel and trade.
"Those are two of the exact measures that that group has asked for and that is exactly what is being done, and clearly we need to have better communicated that."
The International Olympic Committee (IOC) has said it sees no reason to delay or move the Games because of the mosquito-borne disease.
Mr Aylward said that a call to postpone the Games could not be ruled out in the future, but added: "All the information available today... suggests that the games should definitely go ahead."
He restated the WHO's warning that women who are pregnant or seek to get pregnant should not travel to the Zika zone or be exposed to returning partners who may have been infected.
Postponing the games, at this stage, would only "compromise the huge investment that athletes and others have made in preparing for what should be a fantastic occasion."
In their open letter, the 150 scientists said Brazil's mosquito-eradication programme had failed. They cited this, and the country's "weakened" health system, as reasons to postpone or move the Olympics.
In his reply, Rio Mayor Eduardo da Costa Paes said:
In February, the WHO declared recent outbreaks of those diseases in Latin America and French Polynesia a global health emergency requiring a united response.
It said stepping up programmes to eradicate mosquitoes that spread the Zika virus were a priority.
The mayor of Rio said disease-carrying mosquitoes were being eradicated.
The officials were responding to an open letter by scientists saying it was "unethical" for the Games to go ahead.
The letter also said the global health body should revisit its Zika guidance.
The Zika virus is linked to severe birth defects.
Between February and April, Brazil registered more than 90,000 likely cases of Zika. The number of babies born with Zika-linked defects stood at 4,908 in April.
Dr Aylward, who heads the WHO's emergency programme, told the BBC that it was already carrying out a risk-assessment programme "about this disease and the risks it poses both to individuals who get and those who might be subsequently exposed".
In addition, he said, independent experts had reported to the WHO on the implications of the outbreak for travel and trade.
"Those are two of the exact measures that that group has asked for and that is exactly what is being done, and clearly we need to have better communicated that."
The International Olympic Committee (IOC) has said it sees no reason to delay or move the Games because of the mosquito-borne disease.
Mr Aylward said that a call to postpone the Games could not be ruled out in the future, but added: "All the information available today... suggests that the games should definitely go ahead."
He restated the WHO's warning that women who are pregnant or seek to get pregnant should not travel to the Zika zone or be exposed to returning partners who may have been infected.
Postponing the games, at this stage, would only "compromise the huge investment that athletes and others have made in preparing for what should be a fantastic occasion."
In their open letter, the 150 scientists said Brazil's mosquito-eradication programme had failed. They cited this, and the country's "weakened" health system, as reasons to postpone or move the Olympics.
In his reply, Rio Mayor Eduardo da Costa Paes said:
- The city has over 3,000 health officials monitoring the presence of mosquitoes across Rio
- Inspections will be stepped up in August, a time of year when there are anyway fewer mosquitoes
- A team of eradicators will start focusing on Olympic venues a month before the Games.
In February, the WHO declared recent outbreaks of those diseases in Latin America and French Polynesia a global health emergency requiring a united response.
It said stepping up programmes to eradicate mosquitoes that spread the Zika virus were a priority.
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